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Introduction: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over expression of two genes and in combination with recombinant adeno associated viruses (rAAV) has been used to manipulate gene expression in vitro.New Method: To develop a rAAV construct in combination with the viral 2A sequence to allow long term over expression of the vgf gene and fluorescent marker gene for tracking of the transfected neurones in vivo.Results: Transient transfection of the AAV plasmid containing the vgf gene, viral 2A sequence and eGFP into SH SY5Y cells resulted in eGFP fluorescence comparable to a commercially available reporter construct. This increase in fluorescent cells was accompanied by an increase in VGF mRNA expression. Infusion of the rAAV vector containing VGF, viral 2A sequence and eGFP resulted in eGFP fluorescence in the hypothalamus of both mice and Siberian hamsters, 32 weeks post…

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Midlanders have been able to purchase popular Oakley eyewear products from roughly a dozen different retailers for quite some time, but now shoppers have access to more products after the company opened its own store last month.Apparel for both men and women is sold at the store, which takes the place of the former PacSun space. Backpacks, athletic shorts, golf attire, sandals, tank tops and swimwear make up much of the inventory.But sunglasses which is primarily what the Oakley brand is known for nationally are still a major focus at the store. In fact, customers have even more options when they purchase their glasses at the Oakley store.Unlike at other retail locations, shoppers can personally customize their glasses using an iPad. CapEx is the reason I lose money every year on my Hell House. For example, I made about $1,000 in cash flow over the past year. And last week…

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Amuleto is an excellent choice for travelers visiting Zihuatanejo, offering a charming environment alongside many helpful amenities designed to enhance your stay. While staying at Amuleto, visitors can check out Paseo de Pescador (1.4 mi) and Parroquia de Santa Maria de Guadalupe (1.7 mi), some of Zihuatanejo top attractions. Amuleto Hotel offers guests an array of room amenities including air conditioning and a minibar, and getting online is possible, as free wifi is available. Lena: Now, can you pass me my coat, please? Kara: here it comes! Lena: you lasted five seconds. Kara: WAIT, NONONONO THAT NOT PART OF WE WEREN STARTE YOU JUST CHEATED ON Lena: wait to know Alex reaction. Actually, is that your phone? Kara: Yes, why are you as OH NONONONO! Lena: you have me as one of your fave contacts! How cute is that? Oh, here Alex number. Online videos are strong well favored established…

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