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Intercalation of drugs into assembled DNA systems offers versatile new mechanisms for controlled drug delivery. However, current systems are becoming increasingly complex, reducing the practicality of large scale production. Here, we demonstrate a more pragmatic approach where a short DNA sequence was modified with poly(ethylene glycol) (PEG) of various lengths at both 5 termini to provide serum stability and compatibility. The data reveal striking differences in the internalization behaviour and gene silencing efficiency in the tested cell lines, despite their common lung epithelial origins. The model system TMs silencing was lower where clathrin internalization pathway predominated in Calu 3, relative to silencing in H1299 cells where a non clathrin internalization appears dominant. Increased silencing on endosomal disruption was apparent in Calu 3 cells, but absent when cellular internalization was not predominantly clathrin mediated in A549 cells. Splash is a 1984 romantic comedy about a mermaid who leaves the sea to…

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Hoodline partnered with Petfinder, an online adoption site that lists “more than 315,000 adoptable pets from nearly 14,000 animal shelters and rescue groups” to bring you this roundup of dogs near you.(Details like pet availability, training, vaccinations and other features are based on data provided by Petfinder and may be subject to change; contact the shelter for the latest information.)Zeus, border collieZeus is a handsome male border collie dog being kept at Carolina Border Collie Rescue.Zeus wants to be your one and only: He’ll need a home free of other dogs, cats and kids. Zeus is neutered and vaccinated. He has mastered his house training etiquette.About Zeus:Read more about how to adopt Zeus on Petfinder.Zeus is calm, happy, knows lots of tricks and isn’t aggressive at all.Yara, pit bull terrierYara is a charming female pit bull terrier dog currently residing at Independent Animal Rescue Inc.She is already house trained. Plasma…

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